| Gene | Ap1b1 |
| Chromosome | 11 |
| Deletion Start | 5,041,863 |
| Deletion End | 5,009,559 |
| Deletion Size | 32,305 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,010,430 | 5,010,453 | GCTTAGTTTGGTAAGACAGCCTTG |
| TUP | 5,010,456 | 5,010,483 | ACATGTGCTGGATCTCAGCTTGGGAGGC |
| TUR | 5,010,524 | 5,010,547 | CAGGTTAGGCTTGAACTTGCTATG |
| TDF | 5,041,032 | 5,041,051 | TGGTCTTGGCCTTGCTTCAG |
| TDP | 5,041,052 | 5,041,071 | CCTGATGCTCCCAGGGCCCA |
| TDR | 5,041,073 | 5,041,097 | CGTTGGTAACTTCCATGCACATCTG |
PCR Primers
| Primer | Sequence |
| SU | ACTGATAGATTCTTCAGTTACC |
| SD | AGGCTCACTGAGGAGACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18289A-A9 | VGB6 | C57BL/6NTac | |
| 18289A-C11 | VGB6 | C57BL/6NTac | |
