| Gene | Bend7 |
| Chromosome | 2 |
| Deletion Start | 4,720,559 |
| Deletion End | 4,718,235 |
| Deletion Size | 2,325 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,718,754 | 4,718,777 | CGAAGCTCAAGGCAGAAATTGTTG |
| TUP | 4,718,792 | 4,718,821 | TTCCAGTTGAGTGATTTGGGTGGAAAGGTA |
| TUR | 4,718,844 | 4,718,865 | TGATGCGACACTTGTCTGACTG |
| TDF | 4,719,745 | 4,719,763 | GCTGTTGAGAAGGGACCTC |
| TDP | 4,719,769 | 4,719,794 | TCATACCTGGGTGGTCCTGGTTTGTA |
| TDR | 4,719,822 | 4,719,844 | CATCTTGGGATCTCGTGTGGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTCCCAACGGAAAGTTG |
| SD | ATGCATTAAGCAATGGCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18290A-H5 | VGB6 | C57BL/6NTac | |
