| Gene | Relt |
| Chromosome | 7 |
| Deletion Start | 100,853,108 |
| Deletion End | 100,847,201 |
| Deletion Size | 5,908 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,852,517 | 100,852,541 | TGATCTTTCTGCTGTAATTCTGGAC |
| TUP | 100,852,489 | 100,852,515 | CAGGAAGAACAAGCCAACGTCTCCGAG |
| TUR | 100,852,451 | 100,852,469 | GCCTGGCTCCCAACAACTG |
| TDF | 100,848,089 | 100,848,108 | TCCCACCCTTCTGCCTACTG |
| TDP | 100,848,063 | 100,848,087 | ATCCAGGGCTCCCAAGGCTAGTGCC |
| TDR | 100,848,017 | 100,848,036 | ACCTGCCCACAGACAAGATG |
PCR Primers
| Primer | Sequence |
| SU | TAAGCCCAGCTCAGCTCTTC |
| SD | ATAAGCCCAGGATTGGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18296A-A5 | VGB6 | C57BL/6NTac | |
| 18296A-A9 | VGB6 | C57BL/6NTac | |
| 18296A-H6 | VGB6 | C57BL/6NTac | |
| 18296A-E10 | VGB6 | C57BL/6NTac | |
| 18296A-D10 | VGB6 | C57BL/6NTac | |
