| Gene | Mapre3 |
| Chromosome | 5 |
| Deletion Start | 30,865,282 |
| Deletion End | 30,861,817 |
| Deletion Size | 3,466 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,862,524 | 30,862,545 | ATGCTCTTCCCTGGCTGTGTTC |
| TUP | 30,862,547 | 30,862,572 | CTTGAGGAAGGTCAAGTTCCAGGCCA |
| TUR | 30,862,593 | 30,862,613 | TTGCAGCACCTTGAAGTTGTG |
| TDF | 30,864,902 | 30,864,924 | CAGGAACATGAGAGCGAGAACAG |
| TDP | 30,864,926 | 30,864,949 | CCCGTCATCTCGGGCATCATTGGC |
| TDR | 30,864,955 | 30,864,973 | GCTCACCTCCGTGGCATAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTCACAGCCAAGCCTAAG |
| SD | GGTAAAGTGCAAGGGCAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18303A-A5 | VGB6 | C57BL/6NTac | |
| 18303A-C11 | VGB6 | C57BL/6NTac | |
| 18303A-D10 | VGB6 | C57BL/6NTac | |
| 18303A-E10 | VGB6 | C57BL/6NTac | |
| 18303A-F1 | VGB6 | C57BL/6NTac | |
| 18303A-H3 | VGB6 | C57BL/6NTac | |
