| Gene | Rqcd1 |
| Chromosome | 1 |
| Deletion Start | 74,528,856 |
| Deletion End | 74,506,441 |
| Deletion Size | 22,416 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,506,702 | 74,506,720 | CCCAGCAGGCCATTCATTG |
| TUP | 74,506,722 | 74,506,743 | TTCCTTTGCGCCTGGCGTCAGT |
| TUR | 74,506,783 | 74,506,802 | GCCGTGGCAGTATCCTCATC |
| TDF | 74,528,656 | 74,528,679 | ATGGGCTTATGTGTTGTCTTTCTG |
| TDP | 74,528,683 | 74,528,707 | TAGGGCACGTGAAGCACTCAGACAG |
| TDR | 74,528,729 | 74,528,747 | CCTGGGCAAAGGTTGTGTC |
PCR Primers
| Primer | Sequence |
| SU | CCAGTAGTTTGCAGACGTC |
| SD | AGTTGTCCAGGAAGGCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18304A-B2 | VGB6 | C57BL/6NTac | |
| 18304A-D12 | VGB6 | C57BL/6NTac | |
