| Gene | Mthfd1l |
| Chromosome | 10 |
| Deletion Start | 3,973,257 |
| Deletion End | 4,007,849 |
| Deletion Size | 34,593 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,973,986 | 3,973,966 | GGTGCAGCAGTATCTGGAAAG |
| TUP | 3,974,022 | 3,973,995 | ATATCCGGTGGTGATCTGAGCAGAGTCA |
| TUR | 3,974,063 | 3,974,044 | ACAGCAGTGGCCTAGCAAAC |
| TDF | 4,006,885 | 4,006,866 | CGCTCACCCACTAGGTTCTG |
| TDP | 4,006,912 | 4,006,889 | ACCTCGCCCTGTCTCCACCACCTT |
| TDR | 4,006,936 | 4,006,919 | GACCCAAGCGCACCAATG |
PCR Primers
| Primer | Sequence |
| SU | TTATGAAGACCAAGGGTGGC |
| SD | GCTCATAATATGAACCTGGTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18310A-F7 | VGB6 | C57BL/6NTac | |
