| Gene | Tln1 |
| Chromosome | 4 |
| Deletion Start | 43,556,747 |
| Deletion End | 43,531,916 |
| Deletion Size | 24,832 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,556,093 | 43,556,111 | GACACAGGCAGCCAAACAC |
| TUP | 43,556,062 | 43,556,089 | CCTTCTAGATGAACCACCCTAGCCTTCA |
| TUR | 43,556,035 | 43,556,056 | ACACAGCCAAGGAGAAGGAAAG |
| TDF | 43,532,442 | 43,532,462 | GCCTAATCCAGACCGTCCTTC |
| TDP | 43,532,412 | 43,532,439 | TGAGACTTCCTCCCTTCCTAGTCCTGTG |
| TDR | 43,532,384 | 43,532,406 | GGCTACACAGTGCTAACCATCAG |
PCR Primers
| Primer | Sequence |
| SU | AAATCCCAGGAAAGAATGGG |
| SD | TGGGTTTACAACCCTCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18312B-C7 | VGB6 | C57BL/6NTac | |
| 18312A-C5 | VGB6 | C57BL/6NTac | |
