| Gene | Fam63a |
| Chromosome | 3 |
| Deletion Start | 95,295,777 |
| Deletion End | 95,287,951 |
| Deletion Size | 7,827 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,288,420 | 95,288,439 | TGCTGGGCAGGTAAGATGTC |
| TUP | 95,288,440 | 95,288,467 | CACAGATGTGCCTTCCAAAGATGCTTGC |
| TUR | 95,288,480 | 95,288,502 | GCGTGGTCTTGTAAGGAATTGTC |
| TDF | 95,295,378 | 95,295,399 | CAACAGCAAGCAGTTCAACCTG |
| TDP | 95,295,400 | 95,295,420 | TGCGAACAAGGGCTCCTTCGC |
| TDR | 95,295,440 | 95,295,459 | AGCTCCCTTGCCGACCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTTTCAGGGAAGAAGCCAG |
| SD | CCAAGGTAGGAACGCAAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18317B-B11 | VGB6 | C57BL/6NTac | |
| 18317A-H7 | VGB6 | C57BL/6NTac | |
