| Gene | Ndufb8 |
| Chromosome | 19 |
| Deletion Start | 44,555,398 |
| Deletion End | 44,550,257 |
| Deletion Size | 5,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,555,112 | 44,555,131 | CACTGCGGCTTAGTCCTTTC |
| TUP | 44,555,087 | 44,555,107 | TGGGCGCCTAAAGCACCGTGT |
| TUR | 44,555,065 | 44,555,085 | CAGAGGGATGCAGGTCAGATG |
| TDF | 44,551,139 | 44,551,157 | GCGGCAGTCTTTGCAGTTG |
| TDP | 44,551,115 | 44,551,137 | CTGCCCAGTGCTGCCTCTGAAGG |
| TDR | 44,551,072 | 44,551,092 | GGGCTTAGCAAGGCTTGTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGTGTGAGGACAGAAGGCTG |
| SD | AAGGAAAGTGAATCTTGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18319A-G3 | VGB6 | C57BL/6NTac | |
| 18319A-H8 | VGB6 | C57BL/6NTac | |
| 18319A-D11 | VGB6 | C57BL/6NTac | |
