| Gene | Rgs14 |
| Chromosome | 13 |
| Deletion Start | 55,384,142 |
| Deletion End | 55,369,895 |
| Deletion Size | 14,248 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,370,026 | 55,370,045 | GGAGCAGGCTGGAGAAGTTG |
| TUP | 55,370,048 | 55,370,071 | TACAGACAGCTGCTGGCATTGGGA |
| TUR | 55,370,094 | 55,370,112 | TAGCAGGGCATCTGGAAGG |
| TDF | 55,383,921 | 55,383,941 | GCAGGCAGCAGAATGGTCAAG |
| TDP | 55,383,943 | 55,383,971 | ATTGGTGTAGGTGCTTTGGCCATATCGGC |
| TDR | 55,383,976 | 55,383,994 | TGGAGGGAGCCACAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | TCCTTGGAGCCTTCAGATTG |
| SD | GAACAAGTTGAGGAGGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18320A-A5 | VGB6 | C57BL/6NTac | |
| 18320A-D11 | VGB6 | C57BL/6NTac | |
| 18320A-F10 | VGB6 | C57BL/6NTac | |
| 18320A-D1 | VGB6 | C57BL/6NTac | |
