| Gene | Ccdc111 |
| Chromosome | 8 |
| Deletion Start | 46,610,616 |
| Deletion End | 46,578,899 |
| Deletion Size | 31,718 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,609,950 | 46,609,971 | AACAACATTGACGGGACACCTG |
| TUP | 46,609,925 | 46,609,949 | CTGGGAGGTAGGAGGAGCCTATCCC |
| TUR | 46,609,899 | 46,609,919 | TCGCCTCTCATTGGCTTAGTG |
| TDF | 46,579,540 | 46,579,560 | GTGTTCAGTTGGGAGGCTTGG |
| TDP | 46,579,516 | 46,579,538 | TGGATGGCTACCAAGCTCCAGGG |
| TDR | 46,579,489 | 46,579,509 | CAGTGCTAGGGAGGTGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGATTGAACCAGGATTTCACAG |
| SD | TGAGACCTGCTTATCAGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18323A-E11 | VGB6 | C57BL/6NTac | |
| 18323A-F10 | VGB6 | C57BL/6NTac | |
| 18323A-G9 | VGB6 | C57BL/6NTac | |
