| Gene | Dnase2b |
| Chromosome | 3 |
| Deletion Start | 146,596,397 |
| Deletion End | 146,582,190 |
| Deletion Size | 14,208 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 146,595,535 | 146,595,555 | GATGGTGCCCAGCATTGTTTG |
| TUP | 146,595,507 | 146,595,531 | AACCTTCGTTCACGAGGCAACCCAG |
| TUR | 146,595,480 | 146,595,503 | AGAGACCAATAACCTCCTGTCTAC |
| TDF | 146,582,272 | 146,582,296 | TCCGTTATGGGTTCTGTAAGTAAAC |
| TDP | 146,582,250 | 146,582,271 | TCGGTGAAAGGCCACACCCTCT |
| TDR | 146,582,212 | 146,582,232 | CCAAGGCGAGATGTTCCAGTG |
PCR Primers
| Primer | Sequence |
| SU | GAGTTAACTACATGGGTAGC |
| SD | CACCTGCAAAGGTGACAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18324A-E9 | VGB6 | C57BL/6NTac | |
| 18324A-G11 | VGB6 | C57BL/6NTac | |
