| Gene | Efhd1 |
| Chromosome | 1 |
| Deletion Start | 87,297,759 |
| Deletion End | 87,264,453 |
| Deletion Size | 33,307 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,265,327 | 87,265,349 | CGTCGCCTAGTCAGTTACTCTTC |
| TUP | 87,265,351 | 87,265,373 | AACCGGTGAATGGCAGATGCCTC |
| TUR | 87,265,381 | 87,265,399 | TAGTGGCAGGCACCAGTTC |
| TDF | 87,297,677 | 87,297,695 | AGTGGGTTGCTGGCACTGG |
| TDP | 87,297,703 | 87,297,726 | TTCCGAGATTGATGTCGCCCTGGA |
| TDR | 87,297,729 | 87,297,750 | AAGTTCTTGGCACCTCTGACAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGTTTGCAACCAATTCCC |
| SD | GCACAGCCCAGATGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18325A-C3 | VGB6 | C57BL/6NTac | |
| 18325A-D1 | VGB6 | C57BL/6NTac | |
| 18325A-H2 | VGB6 | C57BL/6NTac | |
