| Gene | Smndc1 |
| Chromosome | 19 |
| Deletion Start | 53,389,419 |
| Deletion End | 53,380,455 |
| Deletion Size | 8,965 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,389,211 | 53,389,232 | CAAGACTCGTAGGCACGCAAAG |
| TUP | 53,389,188 | 53,389,210 | CATCTGAGGAAGGCGCTCCCACG |
| TUR | 53,389,149 | 53,389,169 | TGAGAAGCCTCAGTACCTGTG |
| TDF | 53,381,295 | 53,381,313 | GGAGCGAGAGGACCAGAAG |
| TDP | 53,381,264 | 53,381,291 | AAATGGCAGCAGTTCAACAACAGGGCGT |
| TDR | 53,381,211 | 53,381,233 | GAGCAACAAAGTGAGGAGGGATG |
PCR Primers
| Primer | Sequence |
| SU | GATACTTAAAGAAGAAGCAGG |
| SD | TGTCAAGTGCAAAGCATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18327A-C1 | VGB6 | C57BL/6NTac | |
| 18327A-D9 | VGB6 | C57BL/6NTac | |
| 18327A-H12 | VGB6 | C57BL/6NTac | |
| 18327A-H4 | VGB6 | C57BL/6NTac | |
| 18327A-F6 | VGB6 | C57BL/6NTac | |
| 18327A-D11 | VGB6 | C57BL/6NTac | |
