| Gene | Acbd6 |
| Chromosome | 1 |
| Deletion Start | 155,567,678 |
| Deletion End | 155,558,641 |
| Deletion Size | 9,038 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,558,759 | 155,558,778 | GTTTGGCCGAGCTGTTTGAG |
| TUP | 155,558,779 | 155,558,800 | AAGGCTGCCGCACACGTCCAAG |
| TUR | 155,558,841 | 155,558,861 | ACCTGCTTGAACCTGGCATAC |
| TDF | 155,566,808 | 155,566,828 | TGGGAAGGCAGAGTCATACAG |
| TDP | 155,566,834 | 155,566,858 | TGTTGTGTTGTCAAGGAACGCTGCA |
| TDR | 155,566,885 | 155,566,908 | ATCACCTGGAAATCGTATCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CGTATCTCCATCCCGCAC |
| SD | CTCCAGACCACCCAATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18328A-A10 | VGB6 | C57BL/6NTac | |
| 18328A-G10 | VGB6 | C57BL/6NTac | |
| 18328A-C6 | VGB6 | C57BL/6NTac | |
| 18328A-G1 | VGB6 | C57BL/6NTac | |
| 18328A-C10 | VGB6 | C57BL/6NTac | |
