| Gene | Dph1 |
| Chromosome | 11 |
| Deletion Start | 75,190,456 |
| Deletion End | 75,178,938 |
| Deletion Size | 11,519 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,189,887 | 75,189,907 | TGCTCTCCGTACTAGCACCTG |
| TUP | 75,189,847 | 75,189,876 | AAGCCCATAAGAGATTGGACCTGGCCGAAG |
| TUR | 75,189,822 | 75,189,841 | GCTCGTTGGCTTCCCTACAG |
| TDF | 75,179,766 | 75,179,785 | CCACGCCTCTCCATTGACTG |
| TDP | 75,179,740 | 75,179,762 | TTCAGCCTTTCCCAAGCCACTGC |
| TDR | 75,179,679 | 75,179,703 | CCCTCCCTCAACAGGAAATACAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGGTAGCTGGGCTGGTAG |
| SD | GCGCTAGGTGAGAACTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18331A-E10 | VGB6 | C57BL/6NTac | |
| 18331A-A5 | VGB6 | C57BL/6NTac | |
