18332 Kctd14 Status: Cancelled
| Gene | Kctd14 |
| Chromosome | 7 |
| Deletion Start | 97,458,247 |
| Deletion End | 97,454,927 |
| Deletion Size | 3,321 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 97,455,199 | 97,455,220 | TGTCACAGTGGAGCTACCTATC |
| TUP | 97,455,224 | 97,455,249 | CACTCTGGGATCCTGAGACACCCTTC |
| TUR | 97,455,284 | 97,455,304 | CACCATGCCTGGCAAGTCTAC |
| TDF | 97,457,685 | 97,457,708 | CCAGGTCAGCTAGACACTACAAAG |
| TDP | 97,457,710 | 97,457,730 | CGCGCAGGGCCGCTTCTTCAT |
| TDR | 97,457,756 | 97,457,776 | GCGCAGGTAGTCCAAGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTAAGTTTAAGCTGAGATGCC |
| SD | GGCTCAGCTTTAGAACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
