Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

18335 Spire2 Status: ES cell colonies screened / QC positives

Gene	Spire2
Chromosome	8
Deletion Start	123,369,425
Deletion End	123,332,768
Deletion Size	36,658
Genome Build	38

MGI ID	MGI:2446256
NCBI Gene ID	234857
BAC ID	51N12
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	123,333,107	123,333,127	GGCCTCGACCTTTCTCACATC
TUP	123,333,132	123,333,155	CCCAGATAGCGCTCTACCGGACCG
TUR	123,333,174	123,333,195	ACAAGGAGTCCCAGATCCAGAG
TDF	123,369,256	123,369,275	CAGTGACTGCACCAGCTTTG
TDP	123,369,276	123,369,296	TGGCTGATGTCGTGTGCTCCA
TDR	123,369,317	123,369,335	GTCGTGGAGTGGCATTGAG

PCR Primers

Primer	Sequence
SU	GGTTTCTAACAAACCTTAGTCC
SD	AAGCTGCCACACACTCACTG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	89 bp	+	+	-
TDF	TDR	80 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
18335A-D1	VGB6	C57BL/6NTac