| Gene | Trim27 |
| Chromosome | 13 |
| Deletion Start | 21,192,711 |
| Deletion End | 21,180,498 |
| Deletion Size | 12,214 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,181,396 | 21,181,418 | GACTCGGGAGATGGCTTATTGTG |
| TUP | 21,181,425 | 21,181,454 | AAGTGAACTCACAGGACCTTTGTGGAGTGT |
| TUR | 21,181,456 | 21,181,479 | GTGTAGCTGTGCCAGTGTTTATTC |
| TDF | 21,192,140 | 21,192,163 | CGATTCAATCTGTTTCCCTGTGTC |
| TDP | 21,192,165 | 21,192,189 | TGGGCTCTCCATGTTTCATCGCTGG |
| TDR | 21,192,192 | 21,192,214 | CACCTCTACCTCCCAGTAATGTC |
PCR Primers
| Primer | Sequence |
| SU | GTTGCCCGGTGAGCCAGC |
| SD | GAATCTGGCACCTTAAGTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18336A-G8 | VGB6 | C57BL/6NTac | |
| 18336A-H1 | VGB6 | C57BL/6NTac | |
