| Gene | Pif1 |
| Chromosome | 9 |
| Deletion Start | 65,594,837 |
| Deletion End | 65,587,714 |
| Deletion Size | 7,124 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CCCGAGACTTTGTCACCATCAG |
| TUP | | | TCCAGTGCAGCCAGAGGAACTGC |
| TUR | | | AGCGCAGAATCTGGAGCCTTG |
| TDF | | | GGCCTAGCGGTGAATCTTTAC |
| TDP | | | ACTCCTTTCGAGTGGAGGCAGAAGAGA |
| TDR | | | ACACTCTCCAGGGCCACAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCCTGGGTAGAAGTCCC |
| SD | CTCTCCAGCCTGCAAAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18544B-F3 | VGB6 | C57BL/6NTac | |
| 18544B-F11 | VGB6 | C57BL/6NTac | |
| 18544B-G5 | VGB6 | C57BL/6NTac | |
| 18544B-G11 | VGB6 | C57BL/6NTac | |
