| Gene | Pnpla7 |
| Chromosome | 2 |
| Deletion Start | 25,011,706 |
| Deletion End | 24,976,287 |
| Deletion Size | 35,420 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,976,436 | 24,976,457 | TGGGAGTGGTGACAGGTATTTC |
| TUP | 24,976,459 | 24,976,483 | CTGTCCCGAGCTTGACACTTGTTGC |
| TUR | 24,976,488 | 24,976,509 | CCCACACTGTGAAGAACCATTG |
| TDF | 25,010,914 | 25,010,934 | TCTGGGCACTCACTGTCTTAG |
| TDP | 25,010,935 | 25,010,957 | TCTCAGGGCAGCCCAGGGTTTAG |
| TDR | 25,010,976 | 25,010,994 | CCAGGGTATCGGGCACAAC |
PCR Primers
| Primer | Sequence |
| SU | TTGATCCCTGACTGTGCTGC |
| SD | ATGTACTGACAATGAGACGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18560A-C12 | VGB6 | C57BL/6NTac | |
| 18560A-E5 | VGB6 | C57BL/6NTac | |
| 18560A-G3 | VGB6 | C57BL/6NTac | |
