| Gene | Zzef1 |
| Chromosome | 11 |
| Deletion Start | 72,924,717 |
| Deletion End | 72,796,300 |
| Deletion Size | 128,418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGTCGCCAATAAGGTCCTGAAG |
| TUP | | | TTGCTTTGCGGAGGCCAGCC |
| TUR | | | GAGTTCGGACAACGCCATAC |
| TDF | | | GCTCTTTCCCTCCATAGGCTCTG |
| TDP | | | CAGAACATTGCTGCTATCAGCCTGGCC |
| TDR | | | GGTGGCCTTGTTTGGGTAGTTG |
PCR Primers
| Primer | Sequence |
| SU | CTAGACGGATACGTTAACGG |
| SD | CAGGTCTTCAGTGCTGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18561B-G9 | VGB6 | C57BL/6NTac | |
| 18561A-A10 | VGB6 | C57BL/6NTac | |
