| Gene | Htra1 |
| Chromosome | 7 |
| Deletion Start | 130,962,172 |
| Deletion End | 130,936,275 |
| Deletion Size | 25,898 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,937,055 | 130,937,077 | TACCAGGGATCTTGGCAATGTTG |
| TUP | 130,937,082 | 130,937,107 | ACATGGCTTTGGGATCGCAAGAACTC |
| TUR | 130,937,120 | 130,937,141 | TCAGCAGAAAGGGAGAAGGAAG |
| TDF | 130,961,432 | 130,961,451 | TCCCTCTATCCCACGTCTTG |
| TDP | 130,961,452 | 130,961,473 | TTCCAGGCCTCAGCACAGGCTC |
| TDR | 130,961,492 | 130,961,513 | CCTGAGGACAAAGGCAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | AAGTTCACAGCCACAGTCCC |
| SD | TCTTTGATGAGACCTGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18566A-C7 | VGB6 | C57BL/6NTac | |
| 18566A-H2 | VGB6 | C57BL/6NTac | |
| 18566A-G3 | VGB6 | C57BL/6NTac | |
