| Gene | Lrrc32 |
| Chromosome | 7 |
| Deletion Start | 98,500,006 |
| Deletion End | 98,494,225 |
| Deletion Size | 5,782 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,494,647 | 98,494,665 | GGCTGGGACCCTGAACTTG |
| TUP | 98,494,667 | 98,494,690 | ACTGCCTGCTAGAATGCTCCTCCC |
| TUR | 98,494,720 | 98,494,741 | ACGGTGGGAGAGGTTTCCTGAG |
| TDF | 98,499,045 | 98,499,064 | TCCTCAACCTCAGCAGAAAC |
| TDP | 98,499,067 | 98,499,090 | CCTGCGATCCTTTGAGGCTCGACA |
| TDR | 98,499,122 | 98,499,142 | CAGCACATTGTGGCTCAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCTCGCCTTCAAACTTGC |
| SD | TGAGGATAGGCATGTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18567A-D5 | VGB6 | C57BL/6NTac | |
| 18567A-H9 | VGB6 | C57BL/6NTac | |
| 18567A-F1 | VGB6 | C57BL/6NTac | |
| 18567A-H3 | VGB6 | C57BL/6NTac | |
| 18567A-G10 | VGB6 | C57BL/6NTac | |
| 18567A-F6 | VGB6 | C57BL/6NTac | |
