| Gene | Spn |
| Chromosome | 7 |
| Deletion Start | 127,137,330 |
| Deletion End | 127,136,131 |
| Deletion Size | 1,200 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,137,215 | 127,137,237 | CATATCACAGCTCCAAGTACCTC |
| TUP | 127,137,190 | 127,137,212 | AAGCCCAGAATGCCAGCCCATCA |
| TUR | 127,137,145 | 127,137,165 | GCTGATGGTCTCCTTCGAGTC |
| TDF | 127,136,318 | 127,136,339 | CCACGCTCACTACGTTCTTCAG |
| TDP | 127,136,294 | 127,136,316 | AGACGGAAGTCTCGCCAGGGCTC |
| TDR | 127,136,266 | 127,136,283 | CCCAGGCTTCAGCTCTTC |
PCR Primers
| Primer | Sequence |
| SU | AACAATGAATGAAGGCCAGG |
| SD | GTTGCCATCCAGGCTCTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18573A-B5 | VGB6 | C57BL/6NTac | |
| 18573A-D1 | VGB6 | C57BL/6NTac | |
| 18573A-H11 | VGB6 | C57BL/6NTac | |
| 18573A-H2 | VGB6 | C57BL/6NTac | |
| 18573A-D12 | VGB6 | C57BL/6NTac | |
