| Gene | Htr1d |
| Chromosome | 4 |
| Deletion Start | 136,443,658 |
| Deletion End | 136,442,465 |
| Deletion Size | 1,194 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,442,932 | 136,442,955 | GGCCGTCTGGATCATCTCTATTTG |
| TUP | 136,442,958 | 136,442,982 | TCTCCATCCCTCCACTCTTCTGGCG |
| TUR | 136,443,002 | 136,443,020 | CCAGGCAGTCGGACATCTC |
| TDF | 136,443,076 | 136,443,100 | CCCATCCATCTTGCTCATTATCCTG |
| TDP | 136,443,109 | 136,443,131 | CATATACGTGGCCGCCCGGAGTC |
| TDR | 136,443,166 | 136,443,184 | AAGCTGTGCCGTGGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGTCAAAGGCGAGAACC |
| SD | GTGGAAACAATTCCTGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18576A-D2 | VGB6 | C57BL/6NTac | |
| 18576A-H9 | VGB6 | C57BL/6NTac | |
