| Gene | Gm6484 |
| Chromosome | 9 |
| Deletion Start | 21,837,152 |
| Deletion End | 21,835,521 |
| Deletion Size | 1,632 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GAAAGAGGCCACCACTGAATC |
| TUP | | | TGGGCTAGAGGACCCTCCAGTTGTA |
| TUR | | | AGAAGGTCACTGAGGCTGGAAC |
| TDF | | | GCCCATGACCTGAGATGGATTC |
| TDP | | | AGGACCCACGCAGTCCTGGTGCT |
| TDR | | | GGCCTCAGTTTCCCTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GCTGTCTGCAGGAAGGAGAC |
| SD | CCTTTGCAGTGTTGAGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18577A-B4 | VGB6 | C57BL/6NTac | |
| 18577A-B7 | VGB6 | C57BL/6NTac | |
| 18577A-H10 | VGB6 | C57BL/6NTac | |
