| Gene | Car12 |
| Chromosome | 9 |
| Deletion Start | 66,752,004 |
| Deletion End | 66,713,852 |
| Deletion Size | 38,153 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGGTCCTGCTTGTTTGTGTC |
| TUP | | | CCCACACTGTGGTGATACTTGTTCTTGGG |
| TUR | | | GGGTCACTCTTGGTCCCTTCTATTG |
| TDF | | | CCATCCAGTCAGGCCATCTG |
| TDP | | | CGACAACTGTCAACTCGGGCTGAGC |
| TDR | | | GCGGAGCCTATCTGCAACAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCCTGTGAGAACTGGCTC |
| SD | TCAGCTTAAGTCATGCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18600A-G5 | VGB6 | C57BL/6NTac | |
| 18600B-E5 | VGB6 | C57BL/6NTac | |
| 18600B-E6 | VGB6 | C57BL/6NTac | |
