| Gene | Nos2 |
| Chromosome | 11 |
| Deletion Start | 78,959,806 |
| Deletion End | 78,922,271 |
| Deletion Size | 37,536 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCCCTGCTTGTAATACTCTAC |
| TUP | | | TGGAGTCTGATGCACCTTCCTTGGTG |
| TUR | | | GATCCTGGGCCTTCCTTGTC |
| TDF | | | TGAGGCAGGAGAGTTGTAAGTTC |
| TDP | | | TCAGTATGGGCTACCTTAGGCTGCA |
| TDR | | | TGGAGCTGATGTTACAGGTATGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGAAGCTTGTGGATGCTTG |
| SD | TATACAGGAAAGGCCCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18705A-B5 | VGB6 | C57BL/6NTac | |
| 18705A-B6 | VGB6 | C57BL/6NTac | |
| 18705A-E1 | VGB6 | C57BL/6NTac | |
| 18705A-F2 | VGB6 | C57BL/6NTac | |
| 18705A-G8 | VGB6 | C57BL/6NTac | |
| 18705A-H10 | VGB6 | C57BL/6NTac | |
