Gene | 4921528I01Rik |
Chromosome | 18 |
Deletion Start | 22,150,468 |
Deletion End | 22,120,531 |
Deletion Size | 29,938 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | | | GCCAGCTCAACCTACTACCAATG |
TUP | | | TGCACTTGATACAGGTAACTTCCTCTGGAC |
TUR | | | GCCCTCTTCTATGAGTCACCAG |
TDF | | | CCGAGTTGAAGAAGAGGATGGAG |
TDP | | | TGCTATCAGAGGCCATCCACCTCA |
TDR | | | TGCTTCTGCTCTGTCAGTTTC |
PCR Primers
Primer | Sequence |
SU | AATTTCATTCTTACACTGCCAG |
SD | CTGAAATAATGCTACAAGTATGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
18724A-A8 | VGB6 | C57BL/6NTac | |
18724A-B8 | VGB6 | C57BL/6NTac | |