18724   4921528I01Rik     Status: ES cell colonies screened / QC positives

Gene4921528I01Rik
Chromosome18
Deletion Start22,150,468
Deletion End22,120,531
Deletion Size29,938
Genome Build38
MGI IDMGI:1918200
NCBI Gene ID70950
BAC ID128M10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 18

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGCCAGCTCAACCTACTACCAATG
TUPTGCACTTGATACAGGTAACTTCCTCTGGAC
TURGCCCTCTTCTATGAGTCACCAG
TDFCCGAGTTGAAGAAGAGGATGGAG
TDPTGCTATCAGAGGCCATCCACCTCA
TDRTGCTTCTGCTCTGTCAGTTTC

PCR Primers

PrimerSequence
SUAATTTCATTCTTACACTGCCAG
SDCTGAAATAATGCTACAAGTATGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18724A-A8VGB6C57BL/6NTac
18724A-B8VGB6C57BL/6NTac