| Gene | Fshb |
| Chromosome | 2 |
| Deletion Start | 107,058,954 |
| Deletion End | 107,057,177 |
| Deletion Size | 1,778 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTGGTGCTGGAGAGCAATC |
| TUP | | | TGCTGCCATAGCTGTGAATTGACCA |
| TUR | | | CGGCACTCTTCCTTCTCTACTG |
| TDF | | | CTCGGCCCAATACCCAGAAAG |
| TDP | | | TGTACCTTCAAGGAGCTGGTATACGAGACC |
| TDR | | | GCACAGCCAGGCAATCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGGTTAAATCATCATGTTGGAG |
| SD | TTTGCAATCATGCCAGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18841A-E7 | VGB6 | C57BL/6NTac | |
