Gene | Lef1 |
Chromosome | 3 |
Deletion Start | 131,115,542 |
Deletion End | 131,111,475 |
Deletion Size | 4,068 |
Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | | | GTGGCAAATGGGCAGGAAAC |
TUP | | | TGGCCTATACCGGACCGGTTTCG |
TUR | | | CCCTCAGAGCCACCGAAAC |
TDF | | | GCAGCATCTCTGATTGTCCTTTG |
TDP | | | CTCCCAGGAAAGCATCCAGACGGA |
TDR | | | GAGTAGGAGGGTCCCTTGTTG |
PCR Primers
Primer | Sequence |
SU | GTGTCCTCGAAGCACAGACC |
SD | CTCTACTTCATTCATCTCAGC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
18886A-A2 | VGB6 | C57BL/6NTac | |
18886A-E9 | VGB6 | C57BL/6NTac | |
18886A-H9 | VGB6 | C57BL/6NTac | |