| Gene | Rrp1 |
| Chromosome | 10 |
| Deletion Start | 78,412,949 |
| Deletion End | 78,400,729 |
| Deletion Size | 12,221 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTGCATTAACAATGGGCCATCTC |
| TUP | | | CCGGTCCCGAAAGTGTTGGGTTTC |
| TUR | | | TGCACCAGCAAGCAGGTATC |
| TDF | | | CTGCCAAAGGTCACTGCATTC |
| TDP | | | CCCTGTCTCTGGCCCAGCTATCTCC |
| TDR | | | GCAGGCTGGTGAGAAGATAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGTTTCGGGTAAAGCTCG |
| SD | TGACTATGGTGGAGGGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18988A-D7 | VGB6 | C57BL/6NTac | |
| 18988A-E6 | VGB6 | C57BL/6NTac | |
| 18988A-G5 | VGB6 | C57BL/6NTac | |
| 18988A-H7 | VGB6 | C57BL/6NTac | |
