| Gene | Rusc1 |
| Chromosome | 3 |
| Deletion Start | 89,092,470 |
| Deletion End | 89,084,416 |
| Deletion Size | 8,055 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CCTGCTCTCCAGACAGTTTC |
| TUP | | | TGTTGCTCTCCTGATTCTTGCTCTGGA |
| TUR | | | GTCAGGGCGTTGCAGTTC |
| TDF | | | AGCCAGTCAGCCAGCCAAG |
| TDP | | | CCCAGCATGTCATTCTGAGGCAGTGA |
| TDR | | | GCATAACGAAGTGTCCCATCC |
PCR Primers
| Primer | Sequence |
| SU | GCATTCTCTGATGACCCTCC |
| SD | AAGAGGCAGACCAACACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18993A-A11 | VGB6 | C57BL/6NTac | |
| 18993A-B12 | VGB6 | C57BL/6NTac | |
| 18993A-C9 | VGB6 | C57BL/6NTac | |
