| Gene | Mlh3 |
| Chromosome | 12 |
| Deletion Start | 85,269,407 |
| Deletion End | 85,235,604 |
| Deletion Size | 33,804 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAGTGCGGGACTTGGAGAAC |
| TUP | | | TTTATGGCTTCCGAGGAGAGGCCTTG |
| TUR | | | CCAGCCATGTCGGCTATACTTG |
| TDF | | | TGTATTCTTAACCTCTGAGCCATC |
| TDP | | | TCTGCAGCCTCTGTTCACATCTGAAATGA |
| TDR | | | CCTATGCACGGCCATTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGAATGCTTTGGAATTGAAGC |
| SD | AAGAGCAGACCAGCGCTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19262A-D5 | VGB6 | C57BL/6NTac | |
| 19262A-F6 | VGB6 | C57BL/6NTac | |
| 19262A-F11 | VGB6 | C57BL/6NTac | |
| 19262A-G10 | VGB6 | C57BL/6NTac | |
