| Gene | Dact2 |
| Chromosome | 17 |
| Deletion Start | 14,203,726 |
| Deletion End | 14,195,601 |
| Deletion Size | 8,126 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGCTACACTGCGTGAAC |
| TUP | | | TTGAGCCACAGGGCACTGTACTGG |
| TUR | | | AGCGGGCAAGACTCCTCAATG |
| TDF | | | ACTGCACCTCACTCTACCACTC |
| TDP | | | CACCATTGCCGAGACCAGCGAGG |
| TDR | | | GAATCGTTGCTGCTGGACTC |
PCR Primers
| Primer | Sequence |
| SU | CATGTACATTGGCGAACCTG |
| SD | AAATGCAAATCTGGTCCGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19323A-A11 | VGB6 | C57BL/6NTac | |
| 19323A-D5 | VGB6 | C57BL/6NTac | |
| 19323A-F7 | VGB6 | C57BL/6NTac | |
| 19323A-G8 | VGB6 | C57BL/6NTac | |
| 19323A-H5 | VGB6 | C57BL/6NTac | |
