| Gene | Cyp4a14 |
| Chromosome | 4 |
| Deletion Start | 115,496,110 |
| Deletion End | 115,487,117 |
| Deletion Size | 8,994 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGGCCTTCTTGCTCAGTC |
| TUP | | | TTTCTGGTGCTGTTCAAGGCAGTCCA |
| TUR | | | ATGGGAAATGCTGGAGGGTCTTG |
| TDF | | | GGTCTCAGGCCACATCTTTC |
| TDP | | | TGTGTCTCTGCTTGGTTCAGGAACTGC |
| TDR | | | GCCACCTTCAGCTCGTTCATAG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGAGAAATGGGAATTGG |
| SD | TTCATGATGTCCATTGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19338A-D2 | VGB6 | C57BL/6NTac | |
| 19338A-F1 | VGB6 | C57BL/6NTac | |
| 19338A-H4 | VGB6 | C57BL/6NTac | |
