| Gene | Nup133 |
| Chromosome | 8 |
| Deletion Start | 123,949,173 |
| Deletion End | 123,899,272 |
| Deletion Size | 49,902 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | ACTTTGGGACAGCCCATGCTAC |
| TUP | | | ACCCTAGGCTGACTTTGCTCAATCCA |
| TUR | | | CCTGAGATGCTGAGATCCTGAC |
| TDF | | | GGAGGAGTTTCAGCCGTCAG |
| TDP | | | AGTGACCGTGTCTTCTGTTTGCAGGCA |
| TDR | | | TCGCAGTAGGTCCGTCAC |
PCR Primers
| Primer | Sequence |
| SU | ACGAAGAACAAGCCTGAACC |
| SD | CCTGCTCTCCAACCTGTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19427A-A6 | VGB6 | C57BL/6NTac | |
| 19427A-C1 | VGB6 | C57BL/6NTac | |
