| Gene | Ppme1 |
| Chromosome | 7 |
| Deletion Start | 100,371,820 |
| Deletion End | 100,327,822 |
| Deletion Size | 43,999 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCCCAGGCCATTGGAGATTG |
| TUP | | | TGTCTGGGCATTCAGAGTGCCACG |
| TUR | | | GGCCAAGGCCAGCATTTC |
| TDF | | | GGCCACTACAAGCATCTGTTCTG |
| TDP | | | CTGGAGGAGGCCCTTGCTTCTCA |
| TDR | | | GAAGAGGCCAACAGGAGTGATAC |
PCR Primers
| Primer | Sequence |
| SU | GGACGCAGGAAGAAACACTC |
| SD | AAATAAGGGCCAGTGTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19472A-C12 | VGB6 | C57BL/6NTac | |
| 19472A-F5 | VGB6 | C57BL/6NTac | |
| 19472A-G1 | VGB6 | C57BL/6NTac | |
| 19472A-H1 | VGB6 | C57BL/6NTac | |
