| Gene | Samd4b |
| Chromosome | 7 |
| Deletion Start | 28,423,628 |
| Deletion End | 28,401,496 |
| Deletion Size | 22,133 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GTACCCAAGCCCGCTTTCTG |
| TUP | | | AGCTCTGCCTGGAGCACTCGCTG |
| TUR | | | AGCACTGTTGGCCTCTGACTC |
| TDF | | | CCTGTTGAGCCTGCTTGGATTG |
| TDP | | | TCCTGTCTGCGGGAACAAGTCCAA |
| TDR | | | AAAGCCCTTCCTGTGCCTAC |
PCR Primers
| Primer | Sequence |
| SU | CATCTAGTAGTTTATCCATGAC |
| SD | GGTAGGAGGTAAGGGAGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19480B-B10 | VGB6 | C57BL/6NTac | |
| 19480B-D4 | VGB6 | C57BL/6NTac | |
| 19480B-D8 | VGB6 | C57BL/6NTac | |
| 19480B-H6 | VGB6 | C57BL/6NTac | |
