| Gene | Wisp3 |
| Chromosome | 10 |
| Deletion Start | 39,163,637 |
| Deletion End | 39,151,170 |
| Deletion Size | 12,468 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TCCTAAGAAGGATCTCAGCGATG |
| TUP | | | TCTTCCTGCTGGCACAGCTGGAG |
| TUR | | | GGGCACAGAGCTATTGAAGAAATG |
| TDF | | | TCTGTGGAATATGCCTGGACAAG |
| TDP | | | AGATGCTGTGTCCCCAACA |
| TDR | | | TGCCACTTAAATGACCCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | CCGGCAGAGAAATCAGAGAG |
| SD | GATCTGATAGACTGTATTGCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 19819A-E9 | VGB6 | C57BL/6NTac | |
| 19819A-F11 | VGB6 | C57BL/6NTac | |
| 19819A-G5 | VGB6 | C57BL/6NTac | |
